Antimicrobial Activity of Protein Hydrolysate from Marine Molluscs Babylonia spirata (Linnaeus, 1758)

Article history: Received on: 22/02/2016 Revised on: 10/03/2016 Accepted on: 08/05/2016 Available online: 28/07/2016 The present study is to investigate the antimicrobial activity of protein hydrolysate of marine water mollusks Babylonia spirata (Linnaeus, 1758). Protein hydrolysate was prepared from tissue of Babylonia spirata by enzymatic hydrolysis. Enzyme digestion were carried out with the enzyme Trypsin. The protein concentration was estimated by Bradford’s method and the protein quantification was done by using SDS PAGE analysis. Antibacterial assay was carried out against four bacterial pathogens by agar well diffusion method and antifungal activity was performed against three human pathogenic fungal strains. 2.6mg/ml protein concentration was estimated by Bradford’s method and 40 to 200 kDa protein bands were resulted in SDS PAGE analysis. In antimicrobial activity, the maximum zone of inhibition was observed against Staphylococcus aureus22.16 +1.04mm at 1000μg/ml concentration and the maximum zone of inhibition was observed in Aspergillus fumigatus13.5+0.5 in 1000μg/ml concentration. These results are signify that the protein hydrolysate of marine molluscs Babylonia spirata express remarkable antimicrobial activity.